The Beer-Lambert Law describes the linear relationship between concentration and the absorption of light in a sample: absorbance is directly proportional to the concentration of the absorbing substance and to the distance the light travels through it (the optical pathlength). This proportionality is what allows concentration to be determined directly from an optical measurement. For … Continued

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As biomanufacturing moves toward higher-titer processes and concentrated mAb formulations, the measurement tools standard in most facilities are reaching their limits. Fixed-wavelength UV photometers — workhorses of chromatography and downstream processing for decades — are not designed for the concentration ranges modern bioprocessing now demands. Knowing where and why those limitations occur is the first … Continued

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