Pharmaceutical & Biotechnology

Chromatographic separation

Chromatography involves passing a mixture of substances dissolved in a mobile phase through a stationary phase, in order to separate the substances of interest. Chromatography requires accurate monitoring and control and repeatability is essential.

The versatility and efficacy of chromatographic techniques have made them essential in both large scale and analytical separations. Chromatography is widely used in bioprocessing to separate protein molecules from concentrated process fluids. The center of a chromatography system is a column, filled with a media specific to the separation being carried out.

Nearly all proteins absorb UV light at 280nm, the primary reason for this is due to the aromatic amino acids, e.g. phenylalanine, tryptophan, tyrosine and histidine.

Molecular Properties Utilized in Chromatographic Separation.

Benefits of a Kemtrak Instrument include:

  • Real time in-line continuous measurement
  • Non-destructive analysis
  • Dual-wavelength measurement
  • Zero dead volume, no hold up
  • Process/Yield Optimization
  • Quality control
  • Protein monitoring
  • Solvent monitoring

Chromatographic Separation Application Note
DCP007-UV Product brochure
DCP007-NIR Product brochure

The Kemtrak DCP007 UV LED photometer uses a long life high performance LED light source with robust fiber optics to provide drift and noise-free measurement with very high precision.

A proprietary dual wavelength four channel measurement technique and advanced digital electronics design allows deep absorbance measurement to 5 AU. A range of shorter optical pathlengths allow for deeper absorbance measurements. Automatic compensation for sample turbidity and/or fouling of the optical windows ensures trouble free operation.

Measurement cells are available in intrinsically safe hygienic TriClamp flanges to custom manifolds with less than 1 µL internal volume for fast and accurate concentration measurement of proteins.

Installing a Kemtrak DCP007-UV photometer, measurement cell at column outlet, allows the presence of proteins to be detected, and therefore collection/pooling to begin.

A Kemtrak photometer can be used to control and optimize size exclusion (gel filtration) chromatography, ion-exchange chromatography and affinity chromatography.

Contact a Kemtrak sales representative for more information.