Pharmaceutical & Biotechnology

Solvent concentration measurement

Chromatography involves passing a mixture of substances dissolved in a mobile phase through a stationary phase, in order to separate the substances of interest. Chromatography requires accurate monitoring and control and repeatability is essential.

The versatility and efficacy of chromatographic techniques have made them essential in both large scale and analytical separations. Chromatography is widely used in bioprocessing to separate protein molecules from concentrated process fluids. The center of a chromatography system is a column, filled with a media specific to the separation being carried out.

Solvent gradient control can significantly enhance peak separation and the process economy of chromatography applications.

An in-line quantitative sensor permits on-line mobile phase assessment, which with controlled feedback can guarantee accurate and reproducible results. Controlled gradients minimize the effects of changing properties of the stock solvents.

Benefits of a Kemtrak Instrument include:

  • Real time in-line continuous measurement
  • Non-destructive analysis
  • Yield Optimization
  • Quality control
  • Dual-wavelength measurement

Chromatographic Solvent Concentration Application Note
DCP007-NIR Product brochure

The Kemtrak DCP007 NIR LED photometer uses a long life high performance LED light source with robust fiber optics to provide drift and noise-free measurement with very high precision.

A proprietary dual wavelength four channel measurement technique and advanced digital electronics design allows deep absorbance measurement to 5 AU. A range of shorter optical pathlengths allow for deeper absorbance measurements. Automatic compensation for sample turbidity and/or fouling of the optical windows ensures trouble free operation.

Kemtrak hygienic measurement cells available with FDA and UPC VI approved sealing materials, convenient zero dead volume design and contain no electronics or moving parts for ease of use.

Using a Kemtrak DCP007-NIR photometer and feedback control allows the formation of accurate and reproducible solvent gradients at any scale regardless of deviation in temperature, composition, and delivery methods of solvents.

These deviations occur commonly in production scale chromatography and can drastically affect gradient accuracy and reproducibility.

The Kemtrak DCP007-NIR is an advanced dual wavelength NIR photometer and can accurately measure water/solvent mixtures.

Contact a Kemtrak sales representative for more information.