Pharmaceutical & Biotechnology

Chromatographic product detection

The versatility and efficacy of chromatographic techniques have made them essential in both large scale and analytical separation. Chromatography is widely used in bioprocessing to separate protein molecules from concentrated process fluids. The center of a chromatography system is a column, filled with a media specific to the separation being carried out.

Chromatography is used for protein separation and purification in biotechnology and can require one or more chromatography steps during downstream processing.
Separation through chromatography requires specialized equipment to ensure maximum yield and purity.

Advances in bioprocessing methods have led to protein products being expressed and concentrated to higher and higher levels. Higher protein concentrations mean higher absorbance and Therefore, UV analyzers need the ability to measure accurately at those absorbance levels.

Benefits of a Kemtrak Instrument include:

  • Real time in-line continuous measurement
  • Non-destructive analysis
  • Yield Optimization
  • Quality control
  • Dual-wavelength measurement
  • Zero dead volume, no hold up

Chromatographic Product Detection Application Note
DCP007-UV Product brochure

The Kemtrak DCP007-UV LED photometer uses a long life high performance LED light source with robust fiber optics to provide drift and noise-free measurement with very high precision.

A proprietary dual wavelength four channel measurement technique and advanced digital electronics design allows deep absorbance measurement to 5 AU. A range of shorter optical pathlengths allow for deeper absorbance measurements. Automatic compensation for sample turbidity and/or fouling of the optical windows ensures trouble free operation.

 

Using a Kemtrak DCP007-UV photometer will deliver accurate, reliable, and repeatable post column UV measurements and are a minimum requirement during chromatographic separation to ensure good protein fraction purity and maximum yields.

Kemtrak hygienic measurement cells available with FDA and UPC VI approved sealing materials, convenient zero dead volume design and contain no electronics or moving parts for ease of use.

Contact a Kemtrak sales representative for more information.